His film debut was in Prizzi's Honor (1985). In July 2006 Tucci made an appearance on the USA Network TV series Monk in a performance that earned him a 2007 Emmy for Outstanding Guest Actor – Comedy Series. debuted on CBS in the 10:00 pm EDT time slot on November 14 2006.
He also was nominated for a Grammy Award for Best Spoken Word Album for Children for The One And Only Shrek. He was nominated for an Academy Award for Best Supporting Actor for performance in The Lovely Bones (2009) and won an Emmy Award for his performance in Winchell.